Indira M. Raman
Professor, Bill and Gayle Cook Chair in Biological Sciences
Ionic Mechanisms of Neuronal Excitability
Information in the nervous system is transmitted by action potentials, which are transient, all-or-none changes in the voltage across the neuronal membrane. Neurons in different parts of the brain produce different patterns of action potentials. For example, in response to an excitatory synaptic stimulus, some cells fire a single action potential whereas others may fire a burst or cluster of action potentials. Still other cells fire action potentials spontaneously, even in the absence of synaptic input. The characteristics of action potentials produced by any cell depend largely on the properties of ion channels that the cell expresses. These ion channels include voltage-gated channels, calcium-gated channels, and neurotransmitter-gated channels.
The research interests of this lab are in examining the biophysical properties of ion channels intrinsic to neurons, with a goal of identifying how the diversity of ion channel families revealed by molecular biological studies may contribute to neuronal specialization. At present, we are studying neurons of the cerebellum, a part of the brain that is involved in the control of motor behavior. The experiments involve electrophysiological patch-clamp recordings from cerebellar neurons that have been isolated from mouse brain, as well as from neurons in cerebellar preparations in which synaptic connections remain intact. Specifically, we are examining how the ionic currents of neurons of the cerebellar nuclei interact to produce spontaneous action potentials, and how this pattern of firing is modified by inhibitory synaptic input from Purkinje neurons of the cerebellar cortex. Such experimental measurements of currents in specific neuronal classes will be important for the development of accurate computer models of neuronal activity, as well as for cellular-level interpretations of systems-level studies of cerebellar function.
A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability. Flourakis M, Kula-Eversole E, Hutchison AL, Han TH, Aranda K, Moose DL, White KP, Dinner AR, Lear BC, Ren D, Diekman CO, Raman IM, and Allada R. Cell. 2015 August 13;162(4):836-848.
Integration of Purkinje Cell Inhibition by Cerebellar Nucleo-Olivary Neurons. Najac M and Raman IM. Journal of Neuroscience. 2015 January 14;35(2):544-549.
Resurgent current of voltage-gated Na+ channels. Lewis AH and Raman IM. Journal of Physiology. 2014 November 15;592(22):4825-4838.
Interactions among DIV voltage-sensor movement, fast inactivation, and resurgent Na current induced by the NaVβ4 open-channel blocking peptide. Lewis AH and Raman IM. Journal of General Physiology. 2013 September;142(3):191-206.
Iberiotoxin-sensitive and -insensitive BK currents in Purkinje neuron somata. Benton MD, Lewis AH, Bant JS, and Raman IM. Journal of Neurophysiology. 2013 May 15;109(10):2528-2541.
Antagonism of Lidocaine Inhibition by Open-Channel Blockers That Generate Resurgent Na Current. Bant JS, Aman TK, and Raman IM. Journal of Neuroscience. 2013 March 13;33(11):4976-4987.
The Hodgkin-Huxley Heritage: From Channels to Circuits. Catterall WA, Raman IM, Robinson HPC, Sejnowski TJ, and Paulsen O. Journal of Neuroscience. 2012 October 10;32(41):14064-14073.
Purkinje neuron synchrony elicits time-locked spiking in the cerebellar nuclei. Person AL and Raman IM. Nature. 2012 January 26;481(7382):502-505.
Cross-Species Conservation of Open-Channel Block by Na Channel β4 Peptides Reveals Structural Features Required for Resurgent Na Current. Lewis AH and Raman IM. Journal of Neuroscience. 2011 August 10;31(32):11527-11536.
Prolonged Postinhibitory Rebound Firing in the Cerebellar Nuclei Mediated by Group I Metabotropic Glutamate Receptor Potentiation of L-Type Calcium Currents. Zheng N and Raman IM. Journal of Neuroscience. 2011 July 13;31(28):10283-10292.
Control of transient, resurgent, and persistent current by open-channel block by Na channel β4 in cultured cerebellar granule neurons. Bant JS and Raman IM. PNAS. 2010 July 6;107(27):12357-12362.
View all publications by Indira M. Raman listed in the National Library of Medicine (PubMed). Current and former IBiS students in blue.