Guillermo A. Ameer Professor of Biomedical Engineering, Surgery

Research Interests

Bioartificial Organ Systems, Cell Delivery and Transplantation, Tissue Engineering

Our research interests include two areas: biotechnology and tissue engineering. In the first area, molecular cloning techniques, surface modification, and engineering principles are being used to design and develop devices that can neutralize the activity of macromolecules in the blood that are implicated in pathologic conditions or deleterious side effects. For example, the specific removal of beta-2-microglobulin from blood is part of an effort to control the concentration of proteins implicated in the formation and stabilization of amyloid deposits that are present in patients with end-stage renal disease. The tissue engineering efforts currently include cardiovascular and orthopaedic applications. In the cardiovascular arena, we are interested in designing and evaluating biodegradable materials that would be conducive to the formation of small-diameter blood vessels and heart valves. Specifically, we want to understand how the mechanical properties of the biomaterial influence cell signaling and tissue growth.

In the orthopaedic arena, we are addressing problems associated with knee injuries, specifically injuries to the meniscus and ligaments. The meniscus is a cartilaginous structure located in the knee and meniscal as well as ligament tears are a common occurrence during sports activities. A truncated or impaired meniscus or ligament can lead to joint malfunction and to osteoarthritis. Tissue engineering, controlled drug delivery, and gene-expression profiling are some of the tools that are being used to investigate novel ways to promote wound healing within the avascular zone of the meniscus. In the case of chronic degeneration of this tissue, cell/biomaterial interactions are studied with the goal of creating a bioartificial meniscus that could potentially be used for transplantations. Our efforts to engineer a ligament focus on understanding how the microarchitecture and mechanical properties of a biomaterial will influence cell signaling and the resulting tissue. We are also developing novel experimental approaches to non-invasively and quantitatively assess the development engineered tissues in real time (in collaboration with Professor Vadim Backman).

Selected Publications

A biodegradable tri-component graft for anterior cruciate ligament reconstruction. Chung EJ, Sugimoto MJ, Koh JL, and Ameer GA. Journal of Tissue Engineering and Regenerative Medicine. 2017 March;11(3):704-712.

Targeting Heparin to Collagen within Extracellular Matrix Significantly Reduces Thrombogenicity and Improves Endothelialization of Decellularized Tissues. Jiang B, Suen R, Wertheim JA, and Ameer GA. Biomacromolecules. 2016 December 12;17(12):3940-3948.

Mechanocompatible Polymer-Extracellular-Matrix Composites for Vascular Tissue Engineering. Jiang B, Suen R, Wang J-J, Zhang ZJ, Wertheim JA, and Ameer GA. Advanced Healthcare Materials. 2016 July 6;5(13):1594-1605.

Biodegradable Elastomers with Antioxidant and Retinoid-like Properties. van Lith R, Wang X, and Ameer GA. ACS Biomaterials Science & Engineering. 2016 February 8;2(2):268-277.

SIRT1 Overexpression Maintains Cell Phenotype and Function of Endothelial Cells Derived from Induced Pluripotent Stem Cells. Jiang B, Jen M, Perrin L, Wertheim JA, and Ameer GA. Stem Cells and Development. 2015 December 1;24(23):2740-2745.

Enabling non-invasive assessment of an engineered endothelium on ePTFE vascular grafts without increasing oxidative stress. Jiang B, Perrin L, Kats D, Meade T, and Ameer G. Biomaterials. 2015 November;69:110-120.

Albumin Hydrogels Formed by Electrostatically Triggered Self-Assembly and Their Drug Delivery Capability. Baler K, Michael R, Szleifer I, and Ameer GA. Biomacromolecules. 2014 October 13;15(10):3625-3633.

Engineering biodegradable polyester elastomers with antioxidant properties to attenuate oxidative stress in tissues. van Lith R, Gregory EK, Yang J, Kibbe MR, and Ameer GA. Biomaterials. 2014 September;35(28):8113-8122.

A Receptor-Based Bioadsorbent to Target Advanced Glycation End Products in Chronic Kidney Disease. Zhang Y, Lapidos KA, Gal-Moscovici A, Sprague SM, and Ameer GA. Artificial Organs. 2014 June;38(6):474-483.

Photo-crosslinked biodegradable elastomers for controlled nitric oxide delivery. Wang Y, Kibbe MR, and Ameer GA. Biomaterials Science. 2013 June 1;1(6):625-632.

Cotransplantation with specific populations of spina bifida bone marrow stem/progenitor cells enhances urinary bladder regeneration. Sharma AK, Bury MI, Fuller NJ, Marks AJ, Kollhoff DM, Rao MV, Hota PV, Matoka DJ, Edassery SL, Thaker H, Sarwark JF, Janicki JA, Ameer GA, and Cheng EY. PNAS. 2013 March 5;110(10):4003-4008.

The blood and vascular cell compatibility of heparin-modified ePTFE vascular grafts. Hoshi RA, Van Lith R, Jen MC, Allen JB, Lapidos KA, and Ameer G. Biomaterials. 2013 January;34(1):30-41.

View all publications by Guillermo Ameer listed in the National Library of Medicine (PubMed). Current and former IBiS students in blue.