Keith E.J. Tyo Assistant Professor of Chemical & Biological Engineering

Research Interests

Microbial Engineering

What we do: Microbes must cope with harsh, rapidly changing environments to survive. To do this, microbes have developed sophisticated mechanisms to (a) sense the changes in the environment, and (b) respond quickly to these changes to protect themselves from harm or capitalize on an opportunity. Our lab seeks to rewire these fundamental input/output relationships to program cells to do useful things for mankind in a paradigm called synthetic biology. Inputs: We study methods to modify existing environmental detection sensors in yeast and modify them to detect new analytes. Outputs: We investigate ways microbes modify their metabolic networks and use these modifications to increase production of a given metabolite.

Why we do it: Synthetic biology offers a disruptive technology that has vast potential to impact the most important facets of our lives. The way we diagnose diseases, the drugs we use, the fuels we put in our cars, the plastic that is used in our potato chip bag; we have the opportunity to improve the sustainability of our lifestyle. And by reducing costs, we can make aspects of our lifestyle available to those who could not otherwise afford it. We hope to offer technical contributions that, simply put, make the world a better place.

Selected Publications

Characterizing and predicting carboxylic acid reductase activity for diversifying bioaldehyde production. Moura M, Pertusi D, Lenzini S, Bhan N, Broadbelt LJ, and Tyo KEJ. Biotechnology and Bioengineering. 2016 May;113(5):944-952.

Exploring De Novo metabolic pathways from pyruvate to propionic acid. Stine A, Zhang M, Ro S, Clendennen S, Shelton MC, Tyo KEJ, and Broadbelt LJ. Biotechnology Progress. 2016 March/April;32(2):303-311.

N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli. Sekar K, Gentile AM, Bostick JW, and Tyo KEJ. PLoS ONE. 2016 February 22;11(2):e0149746.

Evaluating enzymatic synthesis of small molecule drugs. Moura M, Finkle J, Stainbrook S, Greene J, Broadbelt LJ, and Tyo KEJ. Metabolic Engineering. 2016 January;33:138-147.

Efficient searching and annotation of metabolic networks using chemical similarity. Pertusi DA, Stine AE, Broadbelt LJ, and Tyo KEJ. Bioinformatics. 2015 April 1;31(7):1016-1024.

Regulatory effects on central carbon metabolism from poly-3-hydroxybutryate synthesis. Sekar K and Tyo KEJ. Metabolic Engineering. 2015 March;28:180-189.

Blocking endocytotic mechanisms to improve heterologous protein titers in Saccharomyces cerevisiae. Rodríguez-Limas WA, Tannenbaum V, and Tyo KEJ. Biotechnology and Bioengineering. 2015 February;112(2):376-385.

Impact of protein uptake and degradation on recombinant protein secretion in yeastTyo KEJ, Liu Z, Magnusson Y, Petranovic D, and Nielsen J. Applied Microbiology and Biotechnology. 2014 August;98(16):7149-7159.

Statistical Analysis of Molecular Signal Recording. Glaser JI, Zamft BM, Marblestone AH, Moffitt JR, Tyo K, Boyden ES, Church G, and Kording KP. PLoS Computational Biology. 2013 July 18;9(7):e1003145.

Measuring Cation Dependent DNA Polymerase Fidelity Landscapes by Deep Sequencing. Zamft BM, Marblestone AH, Kording K, Schmidt D, Martin-Alarcon D, Tyo K, Boyden ES, and Church G. PLoS ONE. 2012 August 22;7(8):e43876.

Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae. Liu Z, Tyo KEJ, Martínez J, Petranovic D, and Nielsen J. Biotechnology and Bioengineering. 2012 May;109(5):1259-1268.

Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stressTyo KEJ, Liu Z, Petranovic D, and Nielsen J. BMC Biology. 2012 March 1;10:16.

Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae. Hou J, Tyo K, Liu Z, Petranovic D, and Nielsen J. Metabolic Engineering. 2012 March;14(2):120-127.

View all publications by Keith Tyo listed in the National Library of Medicine (PubMed). Current and former IBiS students in blue.