Xiaozhong (Alec) Wang Associate Professor of Molecular Biosciences

Research Interests

Genetic Analysis of Protocadherin Diversity in the Central Nervous System

My laboratory is interested in understanding regulatory mechanisms important for mammalian development at both organismal and cellular levels. At the organismal level, my laboratory utilizes the mouse as a model system to study the role of transmembrane signaling molecules in the vertebrate neural development. We focus on an important class of neuronal transmembrane signaling molecules known as clustered protocadherins (Pcdh). At the cellular level, we choose mouse embryonic stem cells as our experimental system to investigate the mechanism of how global genetic regulatory mechanisms such as miRNA silencing contributes to the stemness of ES cells and their differentiation. Our general experimental approach is to construct both protein-protein interaction and genetic interaction maps centered on a key node of a regulatory network. We combine both biochemical and genetic approaches to achieve these goals. Unlike other genetic powerhouses such as yeast, worms, and flies, genetic tools in a mammalian system have been poorly developed and the throughput of genetic analysis remains very low. Therefore, while we are approaching specific biological questions, my laboratory is also actively developing new methods and tools to increase the throughput of genetic analysis for vertebrate systems.

Selected Publications

Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping. Voong LN, Xi L, Sebeson AC, Xiong B, Wang J-P, and Wang X. Cell. 2016 December 1;167(6):1555-1570.

Differential Nucleosome Occupancies across Oct4-Sox2 Binding Sites in Murine Embryonic Stem CellsSebeson A, Xi L, Zhang Q, Sigmund A, Wang J-P, Widom J, and Wang X. PLoS ONE. 2015 May 18;10(5):e0127214.

Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant. Izumo M, Pejchal M, Schook AC, Lange RP, Walisser JA, Sato TR, Wang X, Bradfield CA, and Takahashi JS. eLife. 2014 December 19;3:e04617.

A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB. Lu Y, Liang F-X, and Wang X. Molecular Cell.2014 September 4;55(5):758-770.

Improved siRNA/shRNA Functionality by Mismatched Duplex. Wu H, Ma H, Ye C, Ramirez D, Chen S, Montoya J, Shankar P, WangXA, and Manjunath N. PLoS ONE. 2011 December 9;6(12):e28580.

Ago-2-Mediated Slicer Activity Is Essential for Anti-Flaviviral Efficacy of RNAi. Chen S, Chahar HS, Abraham S, Wu H, Pierson TC, Wang XA, and Manjunath N. PLoS ONE. 2011 November 10;6(11):e27551.

Mammalian hyperplastic discs Homolog EDD Regulates miRNA-Mediated Gene Silencing. Su H, Meng S, Lu Y, Trombly MI, Chen J, Lin C, Turk A, and Wang X. Molecular Cell. 2011 July 8;43(1):97-109.

PiggyBac-Based Recessive Screening Method to Identify Pluripotency Regulators. Guo G, Huang Y, Humphreys P, Wang X, and Smith A. PloS ONE. 2011 April 18;6(4):e18189.

Generation of an Inducible Mouse ES Cell Lines Deficient for Argonaute Proteins. Su H and Wang X. Methods in Molecular Biology. 2011;725:295-313.

PDCD10/CCM3 Acts Downstream of γ-Protocadherins to Regulate Neuronal SurvivalLin C, Meng S, Zhu T, and Wang X. Journal of Biological Chemistry. 2010 December 31;285(53):41675-41685.

Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression. Glasmacher E, Hoefig KP, Vogel KU, Rath N, Du L, Wolf C, Kremmer E, Wang X, and Heissmeyer V.Nature Immunology. 2010 August;11(8):725-733.

Disruption of the clock components CLOCK and BMAL1 leads to hypoinsulinaemia and diabetesMarcheva B, Ramsey KM, Buhr ED, Kobayashi Y, Su H, Ko CH, Ivanova G, Omura C, Mo S, Vitaterna MH, Lopez JP, Philipson LH, Bradfield CA, Crosby SD, JeBailey L, Wang X, Takahashi JS, and Bass J. Nature. 2010 July 29;466(7306):627-631.

Gamma-protocadherins regulate the functional integrity of hypothalamic feeding circuitry in mice. Su H, Marcheva B, Meng S, Liang FA, Kohsaka A, Kobayashi Y, Xu AW, Bass J, and Wang X. Developmental Biology. 2010 March 1;339(1):38-50.

Proteomics Analysis Reveals Overlapping Functions of Clustered Protocadherins. Han M-H, Lin C, Meng S, and Wang X.Molecular & Cellular Proteomics. 2010 January 1;9(1):71-83.

View all publications by Xiaozhong Wang listed in the National Library of Medicine (PubMed). Current and former IBiS students in blue.